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TryptoneCAS Number: 91079 40 2 Enzymatic digest of casein Tryptone is a pancreatic digest of casein. Casein is the main protein of milk and is a rich source of amino acid nitrogen. Amongst all amino acids especially Tryptophan is present in high concentrations. Due to the rich nutritional properties, Tryptone is added to media as an accelerator to increase the yield of organisms and is recommended where a rapid and luxuriant growth of micro organisms is

Based on its constituents and the presence of Potassium acetate Sporulation medium is used to induce the induction of spores forming by yeast

35 Suspend 16

Casein hydrolysate

The medium is buffered by Disodium-β-glycerophosphate which prevents a drop in pH by the production of organic acids

15 Suspend 55

If a positive result is obtained after seven days

98 Agar 15 Final pH 7

Chemical formula C5H11NO2S Solubility (20° C) 48 g/l Mol

C17H16N2Na2O6S=422

9 KH2PO4 15 NaCl 2

Terrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E

This formulation supports the growth of most strains that are capable of growing on HL5